Wayne293^® Transfection Medium,Liquid

High-density value-added:

The growth of different HEK293 cells in Wayne293™. Batch, 125mL shake flask, working volume 30mL, ф25mm, 125rpm, humidity 75%, 5% CO2, 37°C for 7 days.  

Continuous passage growth and transient expression of HEK293 F cells in Wayne293™.

125mL shake flask, working volume 30mL, ф25mm, 125rpm, humidity 75%, 5%CO237℃ culture; passage the next day, 20 passages in total, cryopreserved every 5 generations;

unified recovery after passage, and transfection according to the standard method of instruction An IgG plasmid to detect the amount of transient expression.

High-yield expression:

Growth and expression of HEK293 F cells after transfection in Wayne293™ and other commercial media.

Control: Medium F is supplier G's HEK293 transient medium; 125mL shake flask, working volume 30mL,ф25mm, 125rpm, humidity 75%, 5% CO2, 37℃ culture;

according to the standard method of the instruction transfect three expressions respectively Different IgG plasmids were cultured for 7 days.

24 hours after transfection of HEK293F cells in Wayne293 TM and other commercial media