Feature
- Strong adaptability: Designed for HEK 293 suspension cell culture to ensure optimal growth and expression performance.
- High expression: the protein expression of transient transfection was greatly increased
Wayne293® LeExpro Feed Medium
Wayne293® LeTrans Feed Medium is designed to provide sufficient energy and carbon sources for cells to help maintain high energy metabolism and promote protein synthesis. The ingredients in the supplement can regulate the metabolic pathway of cells to achieve short-term and efficient protein expression.
Description
Technical Data
Technical Data
WayneLVPro™ HEK 293 cells were inoculated into LeExpro Medium, domestic medium A, domestic medium B, and imported medium C for Batch culture. The results showed that the highest cell density of LeExpro Medium was 1.07x107 cells/mL, and the cell growth performance was better than other media.
WayneLVPro™ HEK 293 cells were transferred to LeExpro Medium, domestic medium 2 and domestic medium 1 of the original process, and the supplement was the corresponding commercial supplement. High expression and low expression plasmid were selected, and after transfection, the expression levels were harvested for 5 days after culture. The results showed that the protein expression of LeExpro Medium was improved in different degrees in 7 items compared with other medium.
Composition
Composition
| L-glutamine | No |
|---|---|
| Glucose | 75 g/L |
| Phenol red | NO |
| Sodium bicarbonate | NO |
| Hydrolysate | NO |
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